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RT-qPCR HepG2 cells were treated for 24 h and RNA was isolated using the NucleoSpin RNA mini kit (Macherey-Nagel) and reverse transcribed using RT 2 First Strand Kit (SA Biosciences) following the manufacturers instructions

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Because the testing lab carries no commercial stake in the manufacturing outcome, release decisions track the analytical data rather than supplier-side judgement, with acceptance criteria committed in writing before each synthesis run begins

However, histopathology is recommended to differentiate it from other dermatoses.43 Histopathological findings can be polymorphous, with basal layer vacuolation, lymphocyte exocytosis, keratinocyte necrosis, perivascular and/or diffuse lymphocytic infiltrate, with frequent eosinophils (Fig