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The cutoff for the global false discovery rate (FDR) for peptide and protein identification was set to 0.01

doi:10.1152/japplphysiol.91004.2008, PMID: 18845776 Soejima Y, Ostrowski RP, Manaenko A, Fujii M, Tang J, Zhang JH (2012) Hyperbaric oxygen preconditioning attenuates hyperglycemia enhanced hemorrhagic transformation after transient MCAO in rats

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As much as possible, try to match the buffer that your interacting protein source is in with your binding buffer