On the day of assay, the mannitol and sucrose (MAS) buffer (1) was prepared by diluting sterile-filtered MAS 3 in distilled water, resulting in a solution with the following final concentrations: 220 mM mannitol, 70 mM sucrose, 10 mM KH 2 PO 4 , 5 mM MgCl 2 , 2 mM HEPES, 1 mM EGTA and 0.2% w/v fatty-acid free BSA (3117057001) (pH 7.2), and warmed to 37 C
These modifications reduce binding to IGF-binding proteins and enhance stability during laboratory studies
However, given the scarcity of studies, this pattern must be interpreted cautiously
Together, theyre aimed at people who are already training consistently and following a solid nutrition plan, but want an extra edge during a fat-loss phase