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In a 48-well plate, 250 l diluted cells (50,000 cells/well) were combined with 125 l of the transfection complex mixture and incubated for 24 h before removing RNP medium and replacing with DMEM with 10% FBS and 100 units/ml penicillin/streptomycin (15140122
Sequence: Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val Molecular formula: C62H98N16O22 Molar Mass: 1419.55
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In another study, coffee was able to elevate mRNA levels of NQO1 and glutathione-S transferase Al in the liver and the small intestine [57]
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