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LC-MS/MS Fractionated peptides were analyzed using an Easy-nLC 1200 HPLC equipped with a 250mm*75m Easyspray column connected to a Fusion Lumos mass spectrometer (all Thermo Scientific) operating in synchronous precursor selection (SPS)-MS3 mode 38 (10 SPS events)

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Unabsorbed vitamin C acts osmotically in your gastrointestinal tract, drawing water into the intestines and softening stools