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Generation of H 2 O 2 by mitochondria and chloroplasts had been reported by Mehler (1951) but it was assumed that H 2 O 2 formation from O 2 was direct and that the molecule would be removed by enzymes such as catalases, which are now not considered to be found in these organelles
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The column was subjected to gradient program by using water as mobile Phase A and acetonitrile as mobile Phase B
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