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Lysis and solubilization buffer-3 was used as the wash buffer so that the non-specifically bound contaminants are washed out

The growth signal is therefore indirect and paracrine : activating those receptors releases IGF-1, IGF-2, keratinocyte growth factor and nitric oxide , and those mediators drive crypt-cell proliferation, increased villus height, deeper crypts and increased intestinal and portal blood flow [1] [5]

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Addressing root causes is essential for preventing recurrence: Replacing vitamin and mineral deficiencies that result from malabsorption