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Handling & Usage Tips Always wash your hands before handling the vial or syringe to prevent contamination

Ethics declarations Ethics approval and consent to participate Given that this manuscript is a review article and does not involve any direct research on human or animal subjects, ethical approval and consent to participate are not applicable

The CRISPRi-dCpf1 system uses inactivated Cpf1 (dCpf1), which does not generate DSBsthis avoids the lethal issue caused by the low homologous recombination efficiency of Clostridia and achieved 3- to 100-fold gene silencing efficiency in 27 Clostridium strains

Which is why high protein meals should be a priority in your GLP-1 diet