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After glucose depletion in YP20D medium, a feeding solution containing 200 g/L yeast extract, 100 g/L peptone, and 600 g/L glucose was continuously supplied

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While the top of TM6 and ECL3 could not be confidently modelled for the exendin-4 complex, the portion of TM6 and TM7 that were modelled, along with the weak density corresponding to ECL3 supports a backbone conformation more similar to GLP-1 and oxyntomodulin bound receptors, rather than exendin-P5, albeit it is likely that this region is more conformationally dynamic