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Mitochondrial membrane potential measurement Mitochondrial membrane potential was assessed using JC-1 staining, a fluorescent dye that forms aggregates in healthy mitochondria with high membrane potential (red fluorescence) and exists as monomers in depolarized mitochondria with low membrane potential (green fluorescence)

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Gender was not identified as a significant covariate on the apparent clearance of saxagliptin and its active metabolite in the population pharmacokinetic analysis