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Determination of free and total l-carnitine using liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis Serum samples used for the determination of carnitine levels were deproteinized by 0.6 M perchloric acid, neutralized to pH 7 using 1.2 M potassium carbonate, and centrifuged at 1000 g at 4 C for 5 min

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