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For DRG axon regeneration analysis, 12 m thick cryosections were blocked with 5% bovine serum albumin (Sigma-Aldrich, St Louis, MO, USA)/0.1% Triton X-100 in PBS and then incubated with anti--tubulin (1:3000, R&D Systems Tuj1 MAB1195) and SCG10 (1:3000, Novus Biologicals STMN2 NBP1-49461) for 1 h at RT or overnight at 4 C, followed by incubation with secondary antibodies conjugated with Alexa Fluor 488 or 594

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1999;19:RC2626

Effect of trace elements on the seminal oxidative status and correlation to sperm motility in infertile Saudi males