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After cells were harvested, soluble and insoluble fractions were subjected to specialized trypsinization strategies to enhance coverage of proteins with complex tertiary structures (i.e., either filter-aided sample preparation (FASP) digestion, isobaric tags for relative and absolute quantitative (iTRAQ) labeling, or several rounds of high intensity focused ultrasound (HIFU) and pressurization followed by stable isotope labeling (SIL) (S1B Fig)

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