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Finally, epigenetic regulatory networks are incompletely characterized: while individual ubiquitinases, miRNAs, or demethylases are implicated, the integrated epigenetic landscape governing ferroptosis in I/R AKI (e.g., crosstalk between ubiquitination and miRNA-mediated silencing, or epigenetic memory of ferroptosis susceptibility) remains unclear

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Neuromuscular preparation (sciatic nerve - gastrocnemius muscle in situ) the method for studying the muscle response is based on that described by Charlton MP et al