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The science behind this pairing is not complicated once you understand how each compound works independently, and this guide breaks down everything you need to know about combining them safely, timing your doses correctly, and understanding what realistic results actually look like

Cells were grown in Terrific Broth (TB) medium at 37 C until reaching an optical density of 1.2 at 600 nm, then induced at 18 C for 16 h with 0.24 mM isopropyl--D-thiogalactoside (IPTG)

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