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Because all four share one vial, they are reconstituted and dosed together the components cannot be adjusted independently

Briefly, 100 L of cell-free supernatant from each well of a 24-well plate were transferred to a 96-well plate and 20 L of iodonitrotetrazolium chloride (4 mg/mL, Sigma-Aldrich) were added to each well

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doi: 10.1016/j.ejphar.2015.09.024 141 BombaMGranzottoACastelliVOnofrjMLattanzioRCiminiAet al