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We further assessed the delivery of the protein nanodrugs to macrophage cells within the plaques by staining the macrophages using F4/80 antibody

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coli BL21(DE3) containing plasmid pANT43 was inoculated from an overnight preculture at a 1:100 ratio in four 2 l baffled Erlenmeyer flasks filled with 400 ml TB medium containing 50 g ml 1 kanamycin sulfate and incubated at 37 C, 140 rpm using a 5 cm shaking diameter for 2.75 h

By integrating fundamental research with clinical evidence, the main conclusions are summarized here