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DP cells will slowly overflow in DMEM/F-12 (Dulbeccos Modified Eagle Medium/Nutrient Mixture F-12), 20% (v/v) fetal bovine serum (FBS) supplemented with 100 units/ml penicillin and 0.1 mg/ml streptomycin (Invitrogen, Carlsbad, CA, and Sigma, St

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Still, the GSH/GSSG ratios a marker of the cellular redox status were better preserved in supercooled storage with E-Sol5 indicating mitigated oxidative stress

Storage and Freeze-Thaw Once it's in solution, refrigerate at 28C (3646F) and keep it out of light