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The membrane was first blocked in 5% (wt./vol.) skim milk powder (Lab M Limited, Heywood, UK) in tris-buffered saline with 0.1% tween20 (TBST) for 1 h at RT, then was probed with the primary antibody (concentrations and manufacturers in Supplementary Table 4) diluted in TBST with 5% BSA for 16 h at 4 C on a rocker
A Quick Primer Before we can dissect the two analogs, we need to understand the system they interact with
Several clinical trials demonstrated that TLK286 was active and safe to use in combination treatment regimens with standard chemotherapeutic agents, including platinums, taxanes, and anthracyclines
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