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Cholinergic neurons of the enteric nervous system, which interact closely with gastrointestinal epithelial cells, as well as ACh signaling in non-neuronal cells such as enteric glia and pancreatic cells, depend on the essential role of thiamine in ACh production and the facilitation of ACh signaling

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Protein-tyrosine nitration is a post-translational chemical modification process for the highly selective non-enzymatic introduction of a nitro group (-NO 2 ) into the side chain by peroxynitrite (ONOO - ), a short-lived biomolecule generated by the rapid reaction of NO with superoxide radicals in vivo 40,41