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Methods 25 , 402408, doi: 10.1006/meth.2001.1262 (2001)

10.1007/s00438-004-1006-8 Mol

Samples were allowed to cool, and 1.375 ml purified water with a resistivity 18.2 M cm from a Milli-Q system (Merck Millipore) was added to make up to a final volume of 1.5 ml