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These results were supported by MD simulations, showing that GSSG could indeed be broken down again into two short-lived molecules (reaction 3 in Fig

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27 The cryopreserved cells were cultured in Iscoves modified Dulbecos medium supplemented with 20% FBS, insulin, selenium, transferrin, 10 ng/ml of interleukin-6, insulin-like growth factor-1 and vascular endothelial growth factor at 5% O 2 for 1 week before sorting primary MM cells