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10.1016/S01688278(15)311740 151 PolyakS

Biomarkers reshaped diagnosis and trial design

So, there is no risk of overdose

Then the membrane was blocked with 5% BSA for 1 h at room temperature, followed by incubating with primary antibodies and IRDye800 flurophore-conjugated antibodies (LI-COR Biosciences, Lincoln, NE, USA)