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A three-point temperature calibration was performed of the cooling stage to verify the accuracy of the recorded temperature for our experiments using n -dodecane, n -undecane, and n -decane 32,51

Antibodies The following rabbit polyclonal antibodies were used: anti-GSTM2 (#ab125,102, Abcam, Cambridge, UK, 1:1000), anti-GSTM2 (#A13,496, ABclonal, Wuhan, China, 1:2000), anti-GAPDH (#CSB-PA00025A0Rb Flarebio Biotech LLC, College Park, MD, USA, 1:1000), anti-CIDEC (#D222114, Sangon Biotech, Shanghai, China, 1:1000), anti-CIDEC (#12287-1-AP, Proteintech, Rocky Hill, NJ, USA, 1:1000), anti-ADRP/perilipin2 (#15294-1-AP, Proteintech), anti-DGAT2 (#bs-12,998R, BIOSS, Beijing, China), anti-DGAT2 (#A13,891, ABclonal, Wuhan, China, 1:2000), anti-GPAT4 (#bs-15,587R, BIOSS), anti-SREBP1 (#14,088-1-AP, Proteintech), and anti-PCYT1A (#bs-11306R, BIOSS), anti-PCYT1A (#A5935, ABclonal, Wuhan, China, 1:1000)

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12 weeks: Roughly 69 mL used