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Across platforms, metabolite measurements demonstrated consistent cross-platform behavior, with 50% of metabolites showing both high correlation and quantitative agreement, and a smaller subset (7%) exhibiting strong correlation but systematic differences in absolute quantification ( Figure S1E)

Alzheimer disease was diagnosed when subjects met the criteria of the National Institute of Neurological and Communicative Disorders and Stroke-Alzheimer's Disease and Related Disorders Association for definite, probable, or possible Alzheimer disease.19 Subjects who had a stroke during the follow-up period were not excluded