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This line should be bowed out to the same degree as the philtral column of the unaffected side to recreate the subtle curve

After 24 h, wells were co-transfected with 1.1 g full-length FLAG-tagged ATXN2 cDNA in a pCDNA3.1 vector and C-terminal 6His-tagged proteases in pCDNA3.1 using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) following the manufacturers instructions

A separate analysis in *Obesity* journal found that patients who maintained GLP-1 therapy long-term kept approximately 80% of their weight loss, while those who stopped experienced rapid metabolic adaptation back to baseline

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