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Strategies to preserve muscle mass, including resistance exercise and adequate protein intake, would be important considerations
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PLC protein has the biggest size among all PLC family members - 230 kDa and consists of several functional domains including core domain (consisting of EF, X and Y subdomains) which possesses an ability to hydrolyze PIP2, pleckstrin-homology PH and C2 domains, GTP-exchanging (CDC25-like) domain at the C-terminus and two Ras-associating domains at N-terminus [4-6]
The purified non-acylated tirzepatideGIPRmini-G s Nb35 complex at a concentration of 14-16 mg/mL was mixed with 100 M non-acylated tirzepatide at 4 C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific)
