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Homocysteine and cardiovascular disease

Cells were pelleted by centrifugation (300 x g , 3 min, room temperature in 15-ml conical tubes (Corning), followed by 21,000 x g , 2 min, room temperature in microcentrifuge tubes) and stored at -80C until further analysis

In high L-arginine-dependent tumors, such as breast cancer (Abdelmagid et al., 2013), and HCC (Kishikawa et al., 2015), increased expression of the transporter CAT-1 ( SLC7A1 ) has been observed, and CAT-1 ( SLC7A1 ) knockdown decreases the viability of cancer cells and induces apoptosis