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In addition, eotaxin, TNF-, and interleukins 4 and 5 levels have previously been found to be elevated in the broncho-alveolar lavage fluid (BALF) of obese mice, particularly in those with central obesity [33]

Then, 9 10 9 particles of the l-EV fraction from HEK293 cells co-expressing FLAG-MIM I-BAR and Halo7-Rac1 were added to cells in a 6 ml DMEM medium containing 10% EV-depleted FBS

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