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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

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Description IGF-DES (Des(1-3) IGF-1) IGF-DES, also known as Des(1-3) IGF-1, is a truncated analog of insulin-like growth factor-1 lacking the first three N-terminal amino acids that researchers have identified as significantly more potent than full-length IGF-1 due to reduced binding to IGF binding proteins (IGFBPs)