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A likely explanation is that the BAs bound to a sequestrant into the intestinal lumen can't be absorbed and hence travel more distally in the GI tract where the complexes are still capable to activate the TGR5 expressing colonic L-cells

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It should however be noted that Cys residues are used for a wide range of functions catalytic, regulatory, structural, and others whereas the related amino acid selenocysteine (Sec, further discussed below) seems to be uniquely dedicated to the catalysis of redox reactions [2932]