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The ECM of the healthy mammary gland includes glycosaminoglycans and proteoglycans (e.g., hyaluronan and versican), fibrillar collagens (e.g., type I and type III collagen), basement membrane proteins (e.g., collagen type IV and laminins), and fibronectin [56]

1 for schematic overview of sample collection and processing procedures.) The roles of these preparation conditions and their respective hypothesis-testing comparisons are detailed in Table 1

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Simplified research hypothesis: 5-Amino-1MQ inhibits NNMT 1-methylnicotinamide production falls nicotinamide and methyl-donor pathways change downstream metabolic measurements may change in selected cell and animal models