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Entero-endocrine-osseous axis : gastrointestinal hormones as substrates for DPP-4 The initial observation that patients receiving long-term parenteral nutrition develop osteoporosis and osteomalacia raised suspicion about the lack of stimulation for the secretion of incretin hormones in this mode of nutrition and a potential connection between these hormones and bone metabolism [42]

The cells were washed twice with DPBS Ca 2+ /Mg 2+ , and fresh RPMI phenol red-free media was added before live cell imaging