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Abbreviations ACP1 - acid phosphatase CAT - catalase FMN - flavin mononucleotide GPX - glutathione peroxidase GSH - plasma reduced glutathione GSSG - oxidised glutathione MHR - methemoglobin reductase SOD - Zn/Cu superoxide dismutase TMR - transmembrane reductase of ferricyanide Methemoglobin reductase (MHR) is an erythrocyte cytosolic system responsible for reactivating haemoglobin's ability to transport oxygen after it has been converted into methemoglobin by reactive oxygen species (Board & Pidcock, 1981

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Then, the gel was transferred to the decolorant for 2 h