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Tamoxifen (1 M and 5 M) were added and allowed to incubate for another 24 h, after which the fluorescence signals were measured at 485 ex , 527 em using in the H4 Synergy plate reader (BioTek Inc., USA)

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Before attributing symptoms to histamine intolerance, other conditions should be excluded according to UK guidelines, including: Food allergies (following BSACI guidance) Irritable bowel syndrome (NICE CG61) Coeliac disease (NICE NG20) Inflammatory bowel disease Red flags that warrant further investigation include unintentional weight loss, gastrointestinal bleeding, persistent vomiting, and iron-deficiency anaemia

This mechanism is independent of both the autonomic nervous system and hyperpolarization-activated cyclic nucleotide-gated channels