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After 12,000 g centrifugation for 30 min, cell lysates were incubated with Ni-NTA agarose beads for 4 h, then wash the beads with wash buffer (20 mM imidazole in lysis buffer), finally elute proteins by elution buffer (300 mM imidazole in lysis buffer)

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Studied in copper peptide signaling models involving extracellular matrix research, collagen-associated pathways, dermal response biology, and tissue-remodeling systems

We tend to be more prone to post-inflammatory pigmentation, so any energy-based treatment needs to be dosed carefully to avoid triggering dark patches