RNA isolation and quantitative reverse transcription-polymerase chain reaction Total RNA was isolated using the ISOGEN reagent (Nippon Gene) and reverse transcribed to cDNA using the ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan)
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Arch Biol Sci 69(3):439448 Austin L, Arthur H, de Niese M, Gurusinghe A, Baker MS (1988) Micromethods in single muscle fibers: 2
A Randomized Controlled Trial of Combination Letrozole and Clomiphene Citrate or Letrozole Alone for Ovulation Induction in Women with Polycystic Ovary Syndrome
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