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2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes

Acknowledgements This work was supported by grants from the Swedish Cancer Fund (Cancerfonden), the Swedish Research Council (VR), Radiumhemmets Forskningsfonder and Karolinska Institutet

Maternal high-fat diet and early life stress differentially modulate spine density and dendritic morphology in the medial prefrontal cortex of juvenile and adult rats

Use small amounts of healthy fats