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[DOI] [PubMed] [Google Scholar] 27.Gaugler T., Klei L., Sanders S.J., Bodea C.A., Goldberg A.P., Lee A.B., Mahajan M., Manaa D., Pawitan Y., Reichert J., et al

The steps applied for RT-qPCR are as follows: The temperature was set to 98 C for 3 min, followed by 45 cycles of 15 s at 95 C, 30 s at the annealing temperature, 30 s at 72 C, and a melting program at a rate of 0.1 C/10 seconds between 60 and 95 C