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To investigate the ROS generation, the cells were cultured in serum-free medium containing high glucose (HG, 45 mM Dglucose) , normal glucose (NG, 5 mM Dglucose) and 1 mM H 2 O 2 for 12 h

EGCG (C 22 H 18 O 11 ) is a catechin flavanol, specifically a gallate ester formed by the condensation of gallic acid with the (3R)-hydroxyl group of ()-epigallocatechin, labeled A, B, C, and D (Figure 2) (36)