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The plates were incubated overnight at 4 C on a rotating table, then inverted, emptied, and washed with TBS (3 300 L)

The results showed that the formulation was well-tolerated, with no significant changes noted in tear film break-up time (TBUT), ocular surface staining, or ocular surface disease index (OSDI) ratings between the two regimens and associated with improvements in patients with moderate DED [100]