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Enzym Microb Technol 44:269273 Lin J, Liao XY, Zhang J, Du GC, Chen J (2009) Enhancement of glutathione production with a tripeptidase-deficient recombinant Escherichia coli

[5] During the reaction, an amino acid residue in the enzymes active site acts as a general base and abstracts a proton from C-3, leading to the formation of the cis-2,3-enediol intermediate