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The anti-endometriosis effects involve plentiful molecular mechanisms and targets, including anti-proliferative (CCNE1, CDK2, and CDK4), anti-inflammatory (PGE2, COX-2, TNF-, NO, IL-1, IL-6, and IL-8), anti-angiogenic (VEGF, matrix metalloproteinase and Ang-1), pro-apoptotic (Bax, Bak, Bcl-2, Bcl-xL), autophagy (Beclin 1, LC3-II/LC3-I), endoplasmic reticulum stress, endoplasmic reticulum apoptotic pathway, mitochondrial apoptotic pathway and caspase cascade, anti-migratory invasion (MMP-2, MMP-9, TIMP1, E-cadherin, N-cadherin, Snail and Slug), regulation of estrogen and progesterone receptors, and anti-fibrotic (E-calmodulin, -SMA, type I collagen, wave proteins and fibronectin)
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If there is presence of enzymatic activity and/or spontaneous oxidation in storage, we would expect to observe lower levels of total GSH and free GSH, and elevated levels of GSSG, in the EDTA-treated sample stored at 4 o C compared to the non-deproteinized EDTA-treated plasma stored at 80 o C
