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Histology of Zebrafish muscle Zebrafish were euthanized by tricaine (MS-222, Sigma) overdose (0.05%) and fixed in 4% paraformaldehyde in phosphate buffer saline (PBS, pH 7.4) for 5 min

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Clin Chim Acta (1998) 275(2):17584.10.1016/S0009-8981(98)00089-8 23 FitzpatrickAMStephensonSTHadleyGRBurwellLPenugondaMSimonDMet alThiol redox disturbances in children with severe asthma are associated with posttranslational modification of the transcription factor nuclear factor (erythroid-derived 2)-like 2

After 3 months of treatment (when the mice reached the early stage of the disease), TRF-treated Q175 mice showed improved motor activity rhythms and sleepwake cycles