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High pH fractionation- TMT labeled, combined, and dried samples were resuspended in 300 L of Buffer A (5% v/v MeCN, 95% v/v H 2 O, 0.1% v/v formic acid) and fractionated by centrifugation using a peptide desalting column (Pierce, #89852)

They do not constantly refresh in a way that would allow for a total color transformation

The high prevalence of hypertension and diabetes in Afro-Caribbeans in the West could be directly related to the incidence of G6PD deficiency in those populations [6]

Slides were counterstained with filtered Mayers hematoxylin (Sigma-Aldrich, Castle Hill, NSW, Australia) for 2 h in an incubator at 37 C