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ELISA-based binding assay: Clear bottom 384-well plates (high-binding #781061, Greiner, Kremsmnster, Austria), nonfat dry milk (#T145.3, CarlRoth, Karlsruhe, Germany), anti-tetramethylrhodamine (TRITC) antibody (#A6397, Invitrogen, Waltham, MA, USA), HRP-conjugated rabbit secondary antibody (#7074S/#7076S, Cell Signaling, Danvers, MA, USA), 3,3,5,5-tetramethylbenzidine substrate (#ES022, Merck, Darmstadt, Germany)

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Pickarts 2018 review references wrinkle volume reductions up to 55.8%