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Then, the membranes were incubated at 4 C overnight with primary antibodies against PKM1 (1:1000, Cell Signaling Technology, 7067), PKM2 (1:1000, Cell Signaling Technology, 4053), Nrf2 (1:1000, Cell Signaling Technology, 12721), -arrestin1 (1:1000, Cell Signaling Technology, 12697), -arrestin2 (1:1000, Cell Signaling Technology, 3857), 3-tubulin (1:1000, Cell Signaling Technology, 4466), -actin (1:1000, Cell Signaling Technology, 4970), Gclc (1:1000, Proteintech, 12601-1-AP), Gclm (1:1000, Proteintech, 14241-1-AP), and GFAP (1:1000, Millipore, MAB360)