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The resulting fluorescence was collected using a 63/1.4 NA oil immersion objective and detected on a 34-channel spectral GaAsP array detector with the gain set at 650 V and split into two ranges of 490632 nm and 641694 nm

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-KG induces mTORC1 activation by enhancing GTP loading of the RagB protein in a PHD-dependent manner, thus promoting cell growth 160,161