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Methods 200125(4):402408 Luo D, Cao S, Li Z, Tang M, Wang C, Mackon E, Huang Z, Pan J, Wu X, Wu Q, Zhang H, Li R, Li X, Li R, Chen P (2022) Methyl-sensitive amplification polymorphism (MSAP) analysis provides insights into the DNA methylation underlying heterosis in kenaf ( Hibiscus Cannabinus L.) drought tolerance