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We observed decreased ROS-scavenging capacity with increased dimerization of the thiol groups in solution (formation of diNAC) in TBHP-induced ARPE-19 cells, providing insight on the balance between GSH/GSSG and the capacity for reduction of oxidative stress

The resulting PCR products were purified using AxyPrep Mag PCR Clean-Up Kit (Axygen, United States), validated by an Agilent 2,100 Bioanalyzer (Agilent Technologies, United States), and quantified using a Qubit 2.0 Fluorometer (Invitrogen, United States)

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